human SEMA3F shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Human SEMA3F.
shAAV-222545
AAV-h-SEMA3F-shRNA
Ready-to-use AAV expressing shRNA for silencing of Human SEMA3F (semaphorin 3F). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
SEMA-IV; SEMA4; SEMAK
Description (Vector)
The semaphorins are a family of proteins that are involved in signaling. All the family members have a secretion signal, a 500-amino acid sema domain, and 16 conserved cysteine residues (Kolodkin et al., 1993 (PubMed 8269517)). Sequence comparisons have grouped the secreted semaphorins into 3 general classes, all of which also have an immunoglobulin domain. The semaphorin III family, consisting of human semaphorin III (SEMA3A; MIM 603961), chicken collapsin, and mouse semaphorins A, D, and E, all have a basic domain at the C terminus. Chicken collapsin contributes to path finding by axons during development by inhibiting extension of growth cones (Luo et al., 1993 (PubMed 8402908)) through an interaction with a collapsin response mediator protein of relative molecular mass 62K (CRMP62) (Goshima et al., 1995 (PubMed 7637782)), a putative homolog of an axonal guidance associated UNC33 gene product (MIM 601168). SEMA3F is a secreted member of the semaphorin III family.(supplied by OMIM, Mar 2008)
Gene ID
6405
Gene Name (Vector)
semaphorin 3F
Gene Symbol
SEMA3F
HGNC ID
HGNC:10728
ORF Size (aa)
2358
ORF Size (bp)
2358 bp
RefSeq ID
NM_004186
RefSeq Synonyms
NM_004186, NM_001318800, NM_001318798, BC042914,
Species
human
UniGene ID
Hs.32981
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.