human RPE65 shRNA silencing AAV

Name
AAV-h-RPE65-shRNA

Cat No
shAAV-221550

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human RPE65.

shAAV-221550

AAV-h-RPE65-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human RPE65 (RPE65, retinoid isomerohydrolase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

BCO3; LCA2; mRPE65; rd12; RP20; sRPE65

Description (Vector)

RPE65 encodes a protein which is located in the retinal pigment epithelium and is involved in the production of 11-cis retinal and in visual pigment regeneration. There are two forms of this protein, a soluble form called sRPE65, and a palmitoylated, membrane-bound form known as mRPE65. mRPE65 serves as the palmitoyl donor for lecithin retinol acyl transferase (LRAT), the enzyme that catalyzes the vitamin A to all trans retinol step of the chromophore regeneration process. Both mRPE65 and sRPE65 also serve as regulatory proteins, with the ratio and concentrations of these molecules playing a role in the inhibition of 11-cis retinal synthesis. Mutations in this gene have been associated with Leber congenital amaurosis type 2 (LCA2) and retinitis pigmentosa.

Gene ID

6121

Gene Name (Vector)

RPE65, retinoid isomerohydrolase

Gene Symbol

RPE65

HGNC ID

HGNC:10294

ORF Size (aa)

1602

ORF Size (bp)

1602 bp

RefSeq ID

NM_000329

RefSeq Synonyms

NM_000329, BC075036, BC075035,

Species

human

UniGene ID

Hs.2133

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.