human RNF111 shRNA silencing AAV

Name
AAV-h-RNF111-shRNA

Cat No
shAAV-221327

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human RNF111.

shAAV-221327

AAV-h-RNF111-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human RNF111 (ring finger protein 111). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

ARK

Description (Vector)

RNF111 is a nuclear RING-domain containing E3 ubiquitin ligase. This protein interacts with the transforming growth factor (TGF) -beta/NODAL signaling pathway by promoting the ubiquitination and proteosomal degradation of negative regulators, like SMAD proteins, and thereby enhances TGF-beta target-gene transcription. As a modulator of the nodal signaling cascade, this gene plays a critical role in the induction of mesoderm during embryonic development. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.

Gene ID

54778

Gene Name (Vector)

ring finger protein 111

Gene Symbol

RNF111

HGNC ID

HGNC:17384

ORF Size (aa)

2958

ORF Size (bp)

2958 bp

RefSeq ID

NM_017610

RefSeq Synonyms

NM_017610, NM_001330331, NM_001270530, NM_001270529, NM_001270528, BC060862, BC010369,

Species

human

UniGene ID

Hs.404423

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.