human PPM1B shRNA silencing AAV

Name 
AAV-h-PPM1B-shRNA
Cat No 
shAAV-219573
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human PPM1B.

shAAV-219573
AAV-h-PPM1B-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PPM1B (protein phosphatase, Mg2+/Mn2+ dependent 1B). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
PP2CB; PP2CBETA; PP2C-beta; PP2C-beta-X; PPC2BETAX; protein phosphatase 1B; protein phosphatase 1B (formerly 2C), magnesium-dependent, beta isoform; Protein phosphatase 2C isoform beta; protein phosphatase 2C-like protein; Ser/Thr protein phosphatase type 2C beta 2 isoform
Description (eCommerce)
Members of the protein phosphatase 2C (PP2C) family of isoenzymes are serine/threonine protein phosphatases that act as monomers, are magnesium or manganese dependent, and are structurally distinct from PP1, PP2A, and PP2B protein phosphatases. There are 14 mammalian PP2C genes that encode for members of the PP2C family of isoenzymes. Members of this family include PP2C alpha (PPM1A), PP2C beta (PPM1B), PP2C gamma (PPM1G), and PP2C delta (PPM1D). PPM1B represents the protein phosphatase 2C (PP2C) beta isoform 1. Six splice variants of the PPM1B have been identified PPM1B activation by lipophilic compounds has been demonstrated to be associated with the induction of apoptosis PPM1B activity has been shown to be involved in cell cycle control and the dephosphorylation of cyclin dependent kinases cdk2 and cdk6.
Description (Vector)
PPM1B is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase has been shown to dephosphorylate cyclin-dependent kinases (CDKs), and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to cause cell-growth arrest or cell death. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional transcript variants have been described, but currently do not represent full-length sequences.
Gene ID
5495
Gene Name (eCommerce)
protein phosphatase, Mg2+/Mn2+ dependent 1B
Gene Name (Vector)
protein phosphatase, Mg2+/Mn2+ dependent 1B
Gene Symbol
PPM1B
HGNC ID
HGNC:9276
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1440
ORF Size (bp)
1440 bp
Protein Name (eCommerce)
Protein phosphatase 1B
RefSeq ID
NM_002706
RefSeq Synonyms
NM_177969, NM_177968, NM_002706, NM_001033557, NM_001033556, BC064381, BC012002,
Research Areas
Cytokine Signaling,Host-Virus Interactions,IFN Signaling,Immunology,Kinase/Phosphatase,MAPK Signaling,Phosphatase,Signal Transduction
Research Areas (Faceted)
signal_transduction,immunology,infectious_disease,immunology,signal_transduction,cell_biology,cell_biology
Species
human
Target Sentence
Members of the protein phosphatase 2C (PP2C) family of isoenzymes are serine/threonine protein phosphatases that act as monomers, are magnesium or manganese dependent, and are structurally distinct from PP1, PP2A, and PP2B protein phosphatases. There are 14 mammalian PP2C genes that encode for members of the PP2C family of isoenzymes. Members of this family include PP2C alpha (PPM1A), PP2C beta (PPM1B), PP2C gamma (PPM1G), and PP2C delta (PPM1D). PPM1B represents the protein phosphatase 2C (PP2C) beta isoform 1. Six splice variants of the PPM1B have been identified PPM1B activation by lipophilic compounds has been demonstrated to be associated with the induction of apoptosis PPM1B activity has been shown to be involved in cell cycle control and the dephosphorylation of cyclin dependent kinases cdk2 and cdk6.
UniGene ID
Hs.416769
UniProt ID (eCommerce)
O75688

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.