Promotional banner reading 'FALL SAVINGS' with expiry date August 31 on an orange background; features a group of people with orange stacked blocks on the right and discounts for services and catalog products.

human POLR2E shRNA silencing AAV

Name 
AAV-h-POLR2E-shRNA
Cat No 
shAAV-219394
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human POLR2E.

shAAV-219394
AAV-h-POLR2E-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human POLR2E (RNA polymerase II subunit E). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
hRPB25; hsRPB5; RPABC1; RPB5; XAP4
Description (Vector)
POLR2E encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11.
Gene ID
5434
Gene Name (Vector)
RNA polymerase II subunit E
Gene Symbol
POLR2E
HGNC ID
HGNC:9192
ORF Size (aa)
633
RefSeq ID
NM_002695
RefSeq Synonyms
NM_002695, NM_001316324, NM_001316323, BC034144, BC004441,
Species
human
UniGene ID
Hs.24301

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.