human POLR2C shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Human POLR2C.
shAAV-219388
AAV-h-POLR2C-shRNA
Ready-to-use AAV expressing shRNA for silencing of Human POLR2C (RNA polymerase II subunit C). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
DNA-directed RNA polymerase II 33 kDa polypeptide; DNA-directed RNA polymerase II subunit C; DNA-directed RNA polymerase II subunit RPB3; hRPB33; hsRPB3; polymerase (RNA) II (DNA directed) polypeptide C, 33kDa; polymerase (RNA) II subunit C; RNA polymerase II subunit 3; RNA polymerase II subunit B3; RPB3; RPB31; RPB33
Description (eCommerce)
RPB3 (RNA polymerase II subunit 3) is a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. It is a component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P19387].
Description (Vector)
POLR2C encodes the third largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a cysteine rich region and exists as a heterodimer with another polymerase subunit, POLR2J. These two subunits form a core subassembly unit of the polymerase. A pseudogene has been identified on chromosome 21.
Gene ID
5432
Gene Name (eCommerce)
RNA polymerase II subunit C
Gene Name (Vector)
RNA polymerase II subunit C
Gene Symbol
POLR2C
HGNC ID
HGNC:9189
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
828
Protein Name (eCommerce)
DNA-directed RNA polymerase II subunit RPB3
RefSeq ID
NM_032940
RefSeq Synonyms
NM_032940, BC028157, BC003159, BC000409,
Research Areas
Cytoskeleton,DNA Damage/Repair,Gene Expression,Host-Virus Interactions,Huntington's Disease,Immunology,mRNA Processing,mRNA Splicing,Neurobiology,Neurodegeneration,RNA Processing,Spliceosome,Transcription Factor/Regulator
Research Areas (Faceted)
cell_biology,genetics,transcription_translation,infectious_disease,neurobiology,immunology
Species
human
Target Sentence
RPB3 (RNA polymerase II subunit 3) is a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. It is a component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P19387].
UniGene ID
Hs.79402
UniProt ID (eCommerce)
P19387
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.