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human POLR1E shRNA silencing AAV

Name 
AAV-h-POLR1E-shRNA
Cat No 
shAAV-219384
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human POLR1E.

shAAV-219384
AAV-h-POLR1E-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human POLR1E (RNA polymerase I subunit E). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
DNA-directed RNA polymerase I subunit E; DNA-directed RNA polymerase I subunit RPA49; PAF53; polymerase (RNA) I associated factor 1; polymerase (RNA) I polypeptide E, 53kDa; polymerase (RNA) I subunit E; PRAF1; RNA polymerase I subunit A49; RNA polymerase I-associated factor 1; RNA polymerase I-associated factor 53
Description (eCommerce)
POLR1E is a DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. It is a component of RNA polymerase I which synthesizes ribosomal RNA precursors and appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF (By similarity) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9GZS1].
Gene ID
64425
Gene Name (eCommerce)
RNA polymerase I subunit E
Gene Name (Vector)
RNA polymerase I subunit E
Gene Symbol
POLR1E
HGNC ID
HGNC:17631
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1260
ORF Size (bp)
1260 bp
Protein Name (eCommerce)
DNA-directed RNA polymerase I subunit RPA49
RefSeq ID
NM_022490
RefSeq Synonyms
NM_022490, NM_001282766, BC014331, BC001337,
Research Areas
Epigenetic Regulation of Gene Expression,Signal Transduction,TNF Signaling
Research Areas (Faceted)
cell_biology,genetics,signal_transduction
Species
human
Target Sentence
POLR1E is a DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. It is a component of RNA polymerase I which synthesizes ribosomal RNA precursors and appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF (By similarity) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9GZS1].
UniGene ID
Hs.591087
UniProt ID (eCommerce)
Q9GZS1

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.