human PIWIL1 shRNA silencing AAV

Name 
AAV-h-PIWIL1-shRNA
Cat No 
shAAV-219010
Availability
6-8 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human PIWIL1.

shAAV-219010
AAV-h-PIWIL1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PIWIL1 (piwi like RNA-mediated gene silencing 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
CT80.1; HIWI; MIWI; PIWI
Description (Vector)
PIWIL1 encodes a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. The encoded protein may play a role as an intrinsic regulator of the self-renewal capacity of germline and hematopoietic stem cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Gene ID
9271
Gene Name (Vector)
piwi like RNA-mediated gene silencing 1
Gene Symbol
PIWIL1
HGNC ID
HGNC:9007
ORF Size (aa)
2586
ORF Size (bp)
2586 bp
RefSeq ID
NM_004764
RefSeq Synonyms
NM_004764, NM_001190971, BC028581,
Species
human
UniGene ID
Hs.405659

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.