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human PARP4 shRNA silencing AAV

Name 
AAV-h-PARP4-shRNA
Cat No 
shAAV-218244
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human PARP4.

shAAV-218244
AAV-h-PARP4-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PARP4 (poly(ADP-ribose) polymerase family member 4). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
193 kDa vault protein; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)-like 1; ADP-ribosyltransferase diphtheria toxin-like 4; ADPRTL1; ARTD4; H5 proline-rich; I-alpha-I-related; p193; PARP-4; PARPL; PARP-related; PARP-related/IalphaI-related H5/proline-rich; PH5P; poly ADP-ribose polymerase 4; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase-like 1; vault poly(ADP-ribose) polymerase; vault protein, 193-kDa; VAULT3; von Willebrand factor A domain containing 5C; VPARP; VWA5C
Description (eCommerce)
PARP4 [poly(ADP-ribose) polymerase family, member 4] catalyzes the formation of ADP-ribose polymers covalently attached to proteins using NAD+ as a substrate. There are 6 mammalian PARP enzymes: PARP1, PARP2, PARP3, PARP4/vPARP, PARP5/Tankyrase-1, and PARP6/Tankyrase-2. PARP4 is also known as vault PARP (vPARP) due to its association with the vault particle, a 13MDa cytoplasmic ribonucleoprotein complex whose barrel-shaped structure resembles arched cathedral ceilings. vPARP has also been identified separate from the vault particle. It has been localized to the nuclear pore, mitotic spindle, and cytoplasm. It also has been shown to associate with telomerase activity. Studies of vPARP knockout mice show an increased susceptibility to carcinogen-induced tumorigenesis.
Description (Vector)
PARP4 encodes poly(ADP-ribosyl)transferase-like 1 protein, which is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. Since this protein is not capable of binding DNA directly, its transferase activity may be activated by other factors such as protein-protein interaction mediated by the extensive carboxyl terminus.
Gene ID
143
Gene Name (eCommerce)
poly(ADP-ribose) polymerase family member 4
Gene Name (Vector)
poly(ADP-ribose) polymerase family member 4
Gene Symbol
PARP4
HGNC ID
HGNC:271
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
5175
ORF Size (bp)
5175 bp
Protein Name (eCommerce)
Poly [ADP-ribose] polymerase 4
RefSeq ID
NM_006437
RefSeq Synonyms
NM_006437, BC167791,
Research Areas
Apoptosis,Cancer,Cell Cycle,Cytoskeleton,DNA Damage/Repair,Immunology,Nuclear Import/Export,Transport
Research Areas (Faceted)
cancer,cell_biology,cell_cycle,genetics,transcription_translation,immunology
Species
human
Target Sentence
PARP4 [poly(ADP-ribose) polymerase family, member 4] catalyzes the formation of ADP-ribose polymers covalently attached to proteins using NAD+ as a substrate. There are 6 mammalian PARP enzymes: PARP1, PARP2, PARP3, PARP4/vPARP, PARP5/Tankyrase-1, and PARP6/Tankyrase-2. PARP4 is also known as vault PARP (vPARP) due to its association with the vault particle, a 13MDa cytoplasmic ribonucleoprotein complex whose barrel-shaped structure resembles arched cathedral ceilings. vPARP has also been identified separate from the vault particle. It has been localized to the nuclear pore, mitotic spindle, and cytoplasm. It also has been shown to associate with telomerase activity. Studies of vPARP knockout mice show an increased susceptibility to carcinogen-induced tumorigenesis.
UniGene ID
Hs.718412
UniProt ID (eCommerce)
Q9UKK3

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.