human NUB1 shRNA silencing AAV

Name
AAV-h-NUB1-shRNA

Cat No
shAAV-217175

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human NUB1.

shAAV-217175

AAV-h-NUB1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human NUB1 (negative regulator of ubiquitin like proteins 1). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

BS4; NUB1L; NYREN18

Description (Vector)

NUB1 encodes a protein that functions as a negative regulator of NEDD8, a ubiquitin-like protein that conjugates with cullin family members in order to regulate vital biological events. The protein encoded by this gene regulates the NEDD8 conjugation system post-transcriptionally by recruiting NEDD8 and its conjugates to the proteasome for degradation. This protein interacts with the product of the AIPL1 gene, which is associated with Leber congenital amaurosis, an inherited retinopathy, and mutations in that gene can abolish interaction with this protein, which may contribute to the pathogenesis. This protein is also known to accumulate in Lewy bodies in Parkinson's disease and dementia with Lewy bodies, and in glial cytoplasmic inclusions in multiple system atrophy, with this abnormal accumulation being specific to alpha-synucleinopathy lesions. Alternative splicing of this gene results in multiple transcript variants.

Gene ID

51667

Gene Name (Vector)

negative regulator of ubiquitin like proteins 1

Gene Symbol

NUB1

HGNC ID

HGNC:17623

ORF Size (aa)

1806

ORF Size (bp)

1806 bp

RefSeq ID

NM_016118

RefSeq Synonyms

NM_016118, NM_001243351, BC046354,

Species

human

UniGene ID

Hs.647082

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.