human NLRP3 shRNA silencing AAV

Name
AAV-h-NLRP3-shRNA

Cat No
shAAV-216802

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human NLRP3.

shAAV-216802

AAV-h-NLRP3-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human NLRP3 (NLR family pyrin domain containing 3). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

AGTAVPRL; AII; AVP; C1orf7; CIAS1; CLR1.1; FCAS; FCAS1; FCU; MWS; NALP3; PYPAF1

Description (Vector)

NLRP3 encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID). Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid.

Gene ID

114548

Gene Name (Vector)

NLR family pyrin domain containing 3

Gene Symbol

NLRP3

HGNC ID

HGNC:16400

ORF Size (aa)

2940

ORF Size (bp)

2940 bp

RefSeq ID

NM_004895

RefSeq Synonyms

NM_183395, NM_004895, NM_001243133, NM_001127462, NM_001127461, NM_001079821, BC143363, BC143362, BC143359, BC117211,

Species

human

UniGene ID

Hs.159483

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.