human NFKBIL2 shRNA silencing AAV

Name 
AAV-h-NFKBIL2-shRNA
Cat No 
shAAV-216656
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human TONSL.

shAAV-216656
AAV-h-NFKBIL2-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human TONSL (tonsoku-like, DNA repair protein). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
ikappaBR; I-kappa-B-related protein; IKBR; inhibitor of kappa B-related protein; NF-kappa-B inhibitor-like protein 2; NFKBIL2; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 2; tonsoku-like protein
Description (eCommerce)
NF-kappa-B inhibitor-like protein 2 (NFKBIL2) is a component of the MMS22L-TONSL complex, a complex that stimulates the recombination-dependent repair of stalled or collapsed replication forks. The MMS22L-TONSL complex is required to maintain genome integrity during DNA replication by promoting homologous recombination-mediated repair of replication fork-associated double-strand breaks. It may act by mediating the assembly of RAD51 filaments on ssDNA. Within the complex, it may act as a scaffold [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96HA7].
Description (Vector)
TONSL is thought to be a negative regulator of NF-kappa-B mediated transcription. The encoded protein may bind NF-kappa-B complexes and trap them in the cytoplasm, preventing them from entering the nucleus and interacting with the DNA. Phosphorylation of this protein targets it for degradation by the ubiquitination pathway, which frees the NF-kappa-B complexes to enter the nucleus.
Gene ID
4796
Gene Name (eCommerce)
tonsoku like, DNA repair protein
Gene Name (Vector)
tonsoku-like, DNA repair protein
Gene Symbol
TONSL
HGNC ID
HGNC:7801
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
3660
ORF Size (bp)
3660 bp
Protein Name (eCommerce)
Tonsoku-like protein
RefSeq ID
NM_013432
RefSeq Synonyms
NM_013432, BC008782,
Research Areas
Cell Cycle,DNA Damage/Repair,DNA Replication,Replication,Transcription Factor/Regulator
Research Areas (Faceted)
cell_biology,cell_cycle,genetics,transcription_translation
Species
human
Target Sentence
NF-kappa-B inhibitor-like protein 2 (NFKBIL2) is a component of the MMS22L-TONSL complex, a complex that stimulates the recombination-dependent repair of stalled or collapsed replication forks. The MMS22L-TONSL complex is required to maintain genome integrity during DNA replication by promoting homologous recombination-mediated repair of replication fork-associated double-strand breaks. It may act by mediating the assembly of RAD51 filaments on ssDNA. Within the complex, it may act as a scaffold [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96HA7].
UniGene ID
Hs.675285
UniProt ID (eCommerce)
Q96HA7

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.