human MTIF2 shRNA silencing AAV

Name
AAV-h-MTIF2-shRNA

Cat No
shAAV-216001

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human MTIF2.

shAAV-216001

AAV-h-MTIF2-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human MTIF2 (mitochondrial translational initiation factor 2). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Description (Vector)

During the initiation of protein biosynthesis, initiation factor-2 (IF-2) promotes the binding of the initiator tRNA to the small subunit of the ribosome in a GTP-dependent manner. Prokaryotic IF-2 is a single polypeptide, while eukaryotic cytoplasmic IF-2 (eIF-2) is a trimeric protein. Bovine liver mitochondria contain IF-2(mt), an 85-kD monomeric protein that is equivalent to prokaryotic IF-2. The predicted 727-amino acid human protein contains a 29-amino acid presequence. Human IF-2(mt) shares 32 to 38% amino acid sequence identity with yeast IF-2(mt) and several prokaryotic IF-2s, with the greatest degree of conservation in the G domains of the proteins. Two transcript variants encoding the same protein have been found for this gene.

Gene ID

4528

Gene Name (Vector)

mitochondrial translational initiation factor 2

Gene Symbol

MTIF2

HGNC ID

HGNC:7441

ORF Size (aa)

2181

ORF Size (bp)

2181 bp

RefSeq ID

NM_002453

RefSeq Synonyms

NM_002453, NM_001321005, NM_001321004, NM_001321003, NM_001321002, NM_001321001, NM_001005369, BC141420,

Species

human

UniGene ID

Hs.149894

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.