human KPNA2 shRNA silencing AAV

Name 
AAV-h-KPNA2-shRNA
Cat No 
shAAV-213339
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human KPNA2.

shAAV-213339
AAV-h-KPNA2-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human KPNA2 (karyopherin subunit alpha 2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
importin subunit alpha-1; importin subunit alpha-2; importin-alpha-P1; IPOA1; karyopherin alpha 2 (RAG cohort 1, importin alpha 1); Karyopherin subunit alpha-2; pendulin; QIP2; RAG cohort protein 1; RCH1; SRP1alpha; SRP1-alpha
Description (eCommerce)
The import of proteins from the cytoplasm to the nucleus involves docking the protein to receptors associated with the nuclear pore complex followed by translocation through the nuclear pore. RCH1 is an adaptor protein that recognizes the nuclear localization signal (NLS) on cargo and binds the karyopherin importin-beta receptor for nuclear import which is regulated by RanGTP.
Description (Vector)
The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination
Gene ID
3838
Gene Name (eCommerce)
karyopherin subunit alpha 2
Gene Name (Vector)
karyopherin subunit alpha 2
Gene Symbol
KPNA2
HGNC ID
HGNC:6395
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1590
ORF Size (bp)
1590 bp
Protein Name (eCommerce)
Importin subunit alpha-1
RefSeq ID
NM_002266
RefSeq Synonyms
NM_002266, NM_001320611, BC146905, BC067848, BC053343, BC005978,
Research Areas
Cell Cycle,Host-Virus Interactions,Immunology,NFAT Signaling,Nuclear Import/Export,Signal Transduction,TNF Signaling,Transport
Research Areas (Faceted)
cell_biology,cell_cycle,genetics,immunology,infectious_disease,signal_transduction
Species
human
Target Sentence
The import of proteins from the cytoplasm to the nucleus involves docking the protein to receptors associated with the nuclear pore complex followed by translocation through the nuclear pore. RCH1 is an adaptor protein that recognizes the nuclear localization signal (NLS) on cargo and binds the karyopherin importin-beta receptor for nuclear import which is regulated by RanGTP.
UniGene ID
Hs.594238
UniProt ID (eCommerce)
P52292

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.