human HYOU1 shRNA silencing AAV

Name
AAV-h-HYOU1-shRNA

Cat No
shAAV-211734

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human HYOU1.

shAAV-211734

AAV-h-HYOU1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human HYOU1 (hypoxia up-regulated 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

150 kDa oxygen-regulated protein; 170 kDa glucose-regulated protein; epididymis secretory sperm binding protein; Grp170; GRP-170; HSP12A; hypoxia up-regulated protein 1; ORP150; ORP-150; oxygen regulated protein (150kD)

Description (eCommerce)

ORP150 belongs to the heat shock protein 70 family that is involved in stress-dependent induction, resulting in the accumulation of this protein in the endoplasmic reticulum (ER) under hypoxic conditions. ORP150 is also thought to play an important role in protein folding and secretion in the ER and is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. Finally, ORP150 has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. [taken from NCBI Entrez Gene (Gene ID: 10525)].

Description (Vector)

HYOU1 belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment found in the 5' UTR is involved in stress-dependent induction, resulting in the accumulation of this protein in the endoplasmic reticulum (ER) under hypoxic conditions. HYOU1 is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site, resulting in a protein that lacks the N-terminal signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol. In rat, this protein localizes to both the ER by a carboxy-terminal peptide sequence and to mitochondria by an amino-terminal targeting signal.

Gene ID

10525

Gene Name (eCommerce)

hypoxia up-regulated 1

Gene Name (Vector)

hypoxia up-regulated 1

Gene Symbol

HYOU1

HGNC ID

HGNC:16931

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

2037

ORF Size (bp)

2037 bp

Protein Name (eCommerce)

Hypoxia up-regulated protein 1

RefSeq ID

NM_006389

RefSeq Synonyms

NM_006389, NM_001130991, BC072436, BC004560,

Research Areas

Hypoxia,Signal Transduction

Research Areas (Faceted)

cell_biology,signal_transduction

Species

human

Target Sentence

UniGene ID

Hs.277704

UniProt ID (eCommerce)

Q9Y4L1

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.