human HNRNPU shRNA silencing AAV

Name
AAV-h-HNRNPU-shRNA

Cat No
shAAV-211387

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human HNRNPU.

shAAV-211387

AAV-h-HNRNPU-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human HNRNPU (heterogeneous nuclear ribonucleoprotein U). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

EIEE54; GRIP120; heterogeneous nuclear ribonucleoprotein U; heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A); hnRNP U; HNRNPU antisense RNA 1; HNRNPU-AS1; HNRPU; nuclear p120 ribonucleoprotein; p120; p120 nuclear protein; pp120; SAFA; SAF-A; Scaffold-attachment factor A; U21.1

Description (eCommerce)

Heterogeneous nuclear ribonucleoprotein U (hnRNP-U) belongs to a group of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs) that bind and complex with heterogeneous nuclear RNA (hnRNA) and play a role in pre-mRNA processing and transport. In addition to its RNA-binding domain, hnRNP-U contains a scaffold-associated region (SAR)-specific bipartite DNA-binding domain. Recent evidence suggests that hnRNP-U influences the expression of several genes by regulating mRNA stability.

Description (Vector)

HNRNPU belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they form complexes with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene contains a RNA binding domain and scaffold-associated region (SAR)-specific bipartite DNA-binding domain. This protein is also thought to be involved in the packaging of hnRNA into large ribonucleoprotein complexes. During apoptosis, this protein is cleaved in a caspase-dependent way. Cleavage occurs at the SALD site, resulting in a loss of DNA-binding activity and a concomitant detachment of this protein from nuclear structural sites. But this cleavage does not affect the function of the encoded protein in RNA metabolism. At least two alternatively spliced transcript variants have been identified for this gene.

Gene ID

3192

Gene Name (eCommerce)

heterogeneous nuclear ribonucleoprotein U

Gene Name (Vector)

heterogeneous nuclear ribonucleoprotein U

Gene Symbol

HNRNPU

HGNC ID

HGNC:5048

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

2421

ORF Size (bp)

2421 bp

Protein Name (eCommerce)

Heterogeneous nuclear ribonucleoprotein U

RefSeq ID

NM_031844

RefSeq Synonyms

NM_031844, NM_004501, BC024767, BC015782, BC003621, BC003367,

Research Areas

Gene Expression,mRNA Processing,mRNA Splicing,RNA Binding,RNA Processing,Spliceosome

Research Areas (Faceted)

genetics,transcription_translation,cell_biology

Species

human

Target Sentence

UniGene ID

Hs.106212

UniProt ID (eCommerce)

Q00839

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.