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human GUCA1A shRNA silencing AAV

Name 
AAV-h-GUCA1A-shRNA
Cat No 
shAAV-210673
Availability
6-8 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human GUCA1A.

shAAV-210673
AAV-h-GUCA1A-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human GUCA1A (guanylate cyclase activator 1A). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
C6orf131; COD3; CORD14; GCAP; GCAP1; GUCA; GUCA1
Description (Vector)
GUCA1A plays a role in the recovery of retinal photoreceptors from photobleaching. In the recovery phase, the phototransduction messeneger cGMP is replenished by retinal guanylyl cyclase-1 (GC1). GC1 is activated by decreasing Ca(2+) concentrations following photobleaching. The protein encoded by this gene, guanylyl cyclase activating protein 1 (GCAP1), mediates the sensitivity of GC1 to Ca(2+) concentrations. GCAP1 promotes activity of GC1 at low Ca(2+) concentrations and inhibits GC1 activity at high Ca(2+) concentrations. Mutations in this gene cause autosomal dominant cone dystrophy (COD3); a disease characterized by reduced visual acuity associated with progressive loss of color vision. Mutations in this gene prohibit the inactivation of RetGC1 at high Ca(2+) concentrations; causing the constitutive activation of RetGC1 and, presumably, increased cell death. GUCA1A is expressed in retina and spermatagonia.
Gene ID
2978
Gene Name (Vector)
guanylate cyclase activator 1A
Gene Symbol
GUCA1A
HGNC ID
HGNC:4678
ORF Size (aa)
606
RefSeq ID
NM_000409
RefSeq Synonyms
NM_001319062, NM_001319061, NM_000409, BC031663,
Species
human
UniGene ID
Hs.92858

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.