human GRB10 shRNA silencing AAV

Name 
AAV-h-GRB10-shRNA
Cat No 
shAAV-210487
Availability
6-8 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human GRB10.

shAAV-210487
AAV-h-GRB10-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human GRB10 (growth factor receptor bound protein 10). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
Grb-10; GRB10 adapter protein; GRB10 adaptor protein; GRB-IR; growth factor receptor-bound protein 10; insulin receptor-binding protein Grb-IR; IRBP; maternally expressed gene 1; MEG1; RSS
Description (eCommerce)
Growth factor receptor-bound protein 10 (GRB10) is a member of a family of adaptor proteins that bind to a variety of growth factor receptors to regulate receptor signaling and mediate signals for a variety of processes such as metabolism, growth, apoptosis, and migration. As a dimer, via its BPS (between PH and SH2) and SH2 (src-homology 2) domains, GRB10 associates with the insulin receptor (IR) as well as the insulin-like growth factor receptor (IGFR). GRB10’s association with the IR has been shown to have inhibitory effects on insulin-induced kinase signaling; however studies have also reported that GRB10 can also have positive effects on IR signaling. It is suggested that GRB10 can serve in cellular functions independent of an interaction with the IR.
Description (Vector)
The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. Overexpression of some isoforms of the encoded protein inhibits tyrosine kinase activity and results in growth suppression. This gene is imprinted in a highly isoform- and tissue-specific manner, with expression observed from the paternal allele in the brain, and from the maternal allele in the placental trophoblasts. Alternatively spliced transcript variants encoding different isoforms have been identified.
Gene ID
2887
Gene Name (eCommerce)
growth factor receptor bound protein 10
Gene Name (Vector)
growth factor receptor bound protein 10
Gene Symbol
GRB10
HGNC ID
HGNC:4564
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1611
ORF Size (bp)
1611 bp
Protein Name (eCommerce)
Growth factor receptor-bound protein 10
RefSeq ID
NM_001001549
RefSeq Synonyms
NM_005311, NM_001001555, NM_001001550, NM_001001549, BC024285,
Research Areas
Cardiovascular,EGFR Signaling,Insulin Signaling,PDGF Signaling,SCF-KIT Signaling,Signal Transduction,VEGF Signaling,Wnt Signaling
Research Areas (Faceted)
cardiovascular,signal_transduction,stem_cell_biology
Species
human
Target Sentence
Growth factor receptor-bound protein 10 (GRB10) is a member of a family of adaptor proteins that bind to a variety of growth factor receptors to regulate receptor signaling and mediate signals for a variety of processes such as metabolism, growth, apoptosis, and migration. As a dimer, via its BPS (between PH and SH2) and SH2 (src-homology 2) domains, GRB10 associates with the insulin receptor (IR) as well as the insulin-like growth factor receptor (IGFR). GRB10’s association with the IR has been shown to have inhibitory effects on insulin-induced kinase signaling; however studies have also reported that GRB10 can also have positive effects on IR signaling. It is suggested that GRB10 can serve in cellular functions independent of an interaction with the IR.
UniGene ID
Hs.164060
UniProt ID (eCommerce)
Q13322

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.