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human GCH1 shRNA silencing AAV

Name 
AAV-h-GCH1-shRNA
Cat No 
shAAV-209738
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human GCH1.

shAAV-209738
AAV-h-GCH1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human GCH1 (GTP cyclohydrolase 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
dystonia 14; DYT14; DYT5; DYT5a; GCH; GTP cyclohydrolase 1; GTP cyclohydrolase I; GTPCH1; GTP-CH-1; GTP-CH-I; guanosine 5'-triphosphate cyclohydrolase I; HPABH4B
Description (eCommerce)
GTP cyclohydrolase I (GCH1) is a member of the GTP cyclohydrolase family. GCH1 is the first and rate-limiting enzyme in tetrahydrobiopterin (BH4) biosynthesis, catalyzing the conversion of GTP into 7,8-dihydroneopterin triphosphate. BH4 is an essential cofactor required by aromatic amino acid hydroxylases as well as nitric oxide synthases [taken from NCBI Entrez Gene (Gene ID: 2643)].
Description (Vector)
GCH1 encodes a member of the GTP cyclohydrolase family. The encoded protein is the first and rate-limiting enzyme in tetrahydrobiopterin (BH4) biosynthesis, catalyzing the conversion of GTP into 7,8-dihydroneopterin triphosphate. BH4 is an essential cofactor required by aromatic amino acid hydroxylases as well as nitric oxide synthases. Mutations in this gene are associated with malignant hyperphenylalaninemia and dopa-responsive dystonia. Several alternatively spliced transcript variants encoding different isoforms have been described; however, not all variants give rise to a functional enzyme.
Gene ID
2643
Gene Name (eCommerce)
GTP cyclohydrolase 1
Gene Name (Vector)
GTP cyclohydrolase 1
Gene Symbol
GCH1
HGNC ID
HGNC:4193
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
753
ORF Size (bp)
753 bp
Protein Name (eCommerce)
GTP cyclohydrolase 1
RefSeq ID
NM_000161
RefSeq Synonyms
NM_001024071, NM_001024070, NM_001024024, NM_000161, BC025415,
Research Areas
Apoptosis,Cancer,Cardiovascular,IFN Signaling,Metabolism/Metabolic Process,Neurobiology,Neurodegeneration,Parkinson's Disease,Signal Transduction
Research Areas (Faceted)
cardiovascular,cancer,cell_biology,immunology,metabolism,neurobiology,signal_transduction
Species
human
Target Sentence
GTP cyclohydrolase I (GCH1) is a member of the GTP cyclohydrolase family. GCH1 is the first and rate-limiting enzyme in tetrahydrobiopterin (BH4) biosynthesis, catalyzing the conversion of GTP into 7,8-dihydroneopterin triphosphate. BH4 is an essential cofactor required by aromatic amino acid hydroxylases as well as nitric oxide synthases [taken from NCBI Entrez Gene (Gene ID: 2643)].
UniGene ID
Hs.86724
UniProt ID (eCommerce)
P30793

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.