Promotional banner reading 'FALL SAVINGS' with expiry date August 31 on an orange background; features a group of people with orange stacked blocks on the right and discounts for services and catalog products.

human DSP shRNA silencing AAV

Name 
AAV-h-DSP-shRNA
Cat No 
shAAV-207358
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human DSP.

shAAV-207358
AAV-h-DSP-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human DSP (desmoplakin). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
250/210 kDa paraneoplastic pemphigus antigen; DCWHKTA; desmoplakin; DP
Description (eCommerce)
Desmosomes are intercellular junctions that tightly link adjacent cells. Desmoplakin is an obligate component of functional desmosomes that anchors intermediate filaments to desmosomal plaques. The N-terminus of desmoplakin is required for localization to the desmosome and interacts with the N-terminal region of plakophilin 1 and plakoglobin. The C-terminus of desmoplakin binds with intermediate filaments. In the mid-region of desmoplakin, a coiled-coiled rod domain is responsible for homodimerization. Mutations in DSP, the gene encoding desmoplakin, are the cause of several cardiomyopathies and keratodermas as well as the autoimmune disease paraneoplastic pemphigus [taken from NCBI Entrez Gene (Gene ID: 1832)].
Description (Vector)
Desmosomes are intercellular junctions that tightly link adjacent cells. Desmoplakin is an obligate component of functional desmosomes that anchors intermediate filaments to desmosomal plaques. The N-terminus of desmoplakin is required for localization to the desmosome and interacts with the N-terminal region of plakophilin 1 and plakoglobin. The C-terminus of desmoplakin binds with intermediate filaments. In the mid-region of desmoplakin, a coiled-coiled rod domain is responsible for homodimerization. Mutations in this gene are the cause of several cardiomyopathies and keratodermas as well as the autoimmune disease paraneoplastic pemphigus.
Gene ID
1832
Gene Name (eCommerce)
desmoplakin
Gene Name (Vector)
desmoplakin
Gene Symbol
DSP
HGNC ID
HGNC:3052
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
6819
ORF Size (bp)
6819 bp
Protein Name (eCommerce)
Desmoplakin
RefSeq ID
NM_004415
RefSeq Synonyms
NM_004415, NM_001319034, NM_001008844, BC140802,
Research Areas
Apoptosis,Cardiac Development,Cardiology,Cardiomyopathy,Cardiovascular,Cytoskeleton,EGFR Signaling,Mitochondrion,Signal Transduction,Translational Control
Research Areas (Faceted)
cardiovascular,cell_biology,developmental_biology,signal_transduction,transcription_translation
Species
human
Target Sentence
Desmosomes are intercellular junctions that tightly link adjacent cells. Desmoplakin is an obligate component of functional desmosomes that anchors intermediate filaments to desmosomal plaques. The N-terminus of desmoplakin is required for localization to the desmosome and interacts with the N-terminal region of plakophilin 1 and plakoglobin. The C-terminus of desmoplakin binds with intermediate filaments. In the mid-region of desmoplakin, a coiled-coiled rod domain is responsible for homodimerization. Mutations in DSP, the gene encoding desmoplakin, are the cause of several cardiomyopathies and keratodermas as well as the autoimmune disease paraneoplastic pemphigus [taken from NCBI Entrez Gene (Gene ID: 1832)].
UniGene ID
Hs.519873
UniProt ID (eCommerce)
P15924

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.