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human DHX16 shRNA silencing AAV

Name 
AAV-h-DHX16-shRNA
Cat No 
shAAV-206960
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human DHX16.

shAAV-206960
AAV-h-DHX16-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human DHX16 (DEAH-box helicase 16). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
ATP-dependent RNA helicase #3; DBP2; DDX16; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 16; DEAD/H box 16; DEAH (Asp-Glu-Ala-His) box polypeptide 16; DEAH-box protein 16; pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16; PRO2014; Prp2; PRP8; PRPF2; putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16; RNA helicase
Description (eCommerce)
DHX16 is a member of the DEAH-box family of proteins that possesses several conserved motifs which include the highly conserved DEAH (Asp-Glu-Ala-His) amino acid sequence motif. The major activity of DEAH-box proteins is to function as ATP-dependent RNA helicases. RNA helicases play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DH16 was originally identified as DBP2 and discovered to be a functional homolog of the yeast RNA helicase and ATPase, Cdc28/Prp68. DHX16 appears to play a key role in nonsense-mediated mRNA decay (NMD) as well as rRNA processing.
Description (Vector)
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a functional homolog of fission yeast Prp8 protein involved in cell cycle progression. This gene is mapped to the MHC region on chromosome 6p21.3, a region where many malignant, genetic and autoimmune disease genes are linked. Two transcript variants encoding different isoforms have been found for this gene.
Gene ID
8449
Gene Name (eCommerce)
DEAH-box helicase 16
Gene Name (Vector)
DEAH-box helicase 16
Gene Symbol
DHX16
HGNC ID
HGNC:2739
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
3129
ORF Size (bp)
3129 bp
Protein Name (eCommerce)
Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16
RefSeq ID
NM_003587
RefSeq Synonyms
NM_003587, NM_001164239, BC009392, BC008825,
Research Areas
Gene Expression,Helicase Activity,mRNA Processing,mRNA Splicing,RNA Processing,Spliceosome
Research Areas (Faceted)
genetics,transcription_translation,cell_biology
Species
human
Target Sentence
DHX16 is a member of the DEAH-box family of proteins that possesses several conserved motifs which include the highly conserved DEAH (Asp-Glu-Ala-His) amino acid sequence motif. The major activity of DEAH-box proteins is to function as ATP-dependent RNA helicases. RNA helicases play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DH16 was originally identified as DBP2 and discovered to be a functional homolog of the yeast RNA helicase and ATPase, Cdc28/Prp68. DHX16 appears to play a key role in nonsense-mediated mRNA decay (NMD) as well as rRNA processing.
UniGene ID
Hs.485060
UniProt ID (eCommerce)
O60231

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.