human DCTN1 shRNA silencing AAV

Name
AAV-h-DCTN1-shRNA

Cat No
shAAV-206672

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human DCTN1.

shAAV-206672

AAV-h-DCTN1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human DCTN1 (dynactin subunit 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

150 kDa dynein-associated polypeptide; DAP-150; DP-150; dynactin 1 (p150, glued homolog, Drosophila); dynactin subunit 1; P135; p150-glued

Description (eCommerce)

p150glued is the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. The p150glued subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus [taken from NCBI Entrez Gene (Gene ID: 1639)].

Description (Vector)

DCTN1 encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA).

Gene ID

1639

Gene Name (eCommerce)

dynactin subunit 1

Gene Name (Vector)

dynactin subunit 1

Gene Symbol

DCTN1

HGNC ID

HGNC:2711

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

3420

ORF Size (bp)

3420 bp

Protein Name (eCommerce)

Dynactin subunit 1

RefSeq ID

NM_004082

RefSeq Synonyms

NM_023019, NM_004082, NM_001190837, NM_001190836, NM_001135041, NM_001135040, BC071583, BC006163,

Research Areas

Amyotrophic Lateral Sclerosis (ALS),Antigen Processing and Presentation,Apoptosis,Cell Cycle,Cytoskeleton,Huntington's Disease,Immunology,Neurobiology,Neurodegeneration,Neurodevelopment,Parkinson's Disease

Research Areas (Faceted)

cell_biology,cell_cycle,developmental_biology,genetics,immunology,neurobiology

Species

human

Target Sentence

UniGene ID

Hs.516111

UniProt ID (eCommerce)

Q14203

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.