human DARS2 shRNA silencing AAV

Name 
AAV-h-DARS2-shRNA
Cat No 
shAAV-206605
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human DARS2.

shAAV-206605
AAV-h-DARS2-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human DARS2 (aspartyl-tRNA synthetase 2, mitochondrial). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
ASPRS; LBSL; MT-ASPRS
Description (Vector)
DARS2 belongs to the class-II aminoacyl-tRNA synthetase family. It is a mitochondrial enzyme that specifically aminoacylates aspartyl-tRNA. Mutations in this gene are associated with leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL).
Gene ID
55157
Gene Name (Vector)
aspartyl-tRNA synthetase 2, mitochondrial
Gene Symbol
DARS2
HGNC ID
HGNC:25538
ORF Size (aa)
1938
ORF Size (bp)
1938 bp
RefSeq ID
NM_018122
RefSeq Synonyms
NM_018122, BC045173,
Species
human
UniGene ID
Hs.647707

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.