human CHEK2 shRNA silencing AAV

Name
AAV-h-CHEK2-shRNA

Cat No
shAAV-205125

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human CHEK2.

shAAV-205125

AAV-h-CHEK2-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human CHEK2 (checkpoint kinase 2). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

CDS1; cds1 homolog; Checkpoint kinase 2; checkpoint-like protein CHK2; CHK2; CHK2 checkpoint homolog; hCds1; HuCds1; LFS2; PP1425; RAD53; serine/threonine-protein kinase Chk2

Description (eCommerce)

Chk2 functions as a checkpoint protein kinase in response to DNA damage to maintain genomic stability. The yeast homologs are RAD53 and Cds1. The two major upstream controllers of Chk2 are ATM and ATR which are part of the phosphatidylinositol 3-kinase family. When activated, Chk2 targets p53, Brca1, Cdc25A and Cdc25C to cause cell cycle arrest. Chk2 is also known as serine/threonine-protein kinase CHK2, CHEK2, CDS1, LFS2, RAD53, HuCds1, and PP1425.

Description (Vector)

In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene.

Gene ID

11200

Gene Name (eCommerce)

checkpoint kinase 2

Gene Name (Vector)

checkpoint kinase 2

Gene Symbol

CHEK2

HGNC ID

HGNC:16627

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

1632

ORF Size (bp)

1632 bp

Protein Name (eCommerce)

Serine/threonine-protein kinase Chk2

RefSeq ID

NM_007194

RefSeq Synonyms

NM_145862, NM_007194, NM_001257387, NM_001005735, BC004207,

Research Areas

Apoptosis,Breast Cancer,Cancer,Cell Cycle,Cell Division,DNA Damage/Repair,Homologous Recombination,Host-Virus Interactions,Kinase/Phosphatase,p53 Signaling,Prostate Cancer,Signal Transduction,Transcription Factor/Regulator,Tumor Suppressors/Oncoproteins

Research Areas (Faceted)

cancer,cell_biology,cell_cycle,genetics,transcription_translation,infectious_disease,signal_transduction

Species

human

Target Sentence

UniGene ID

Hs.505297

UniProt ID (eCommerce)

O96017

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.