human DBT Over-expression AAV

Name 
AAV-h-DBT
Cat No 
AAV-206629
Availability
4-5 weeks
Categories
AAV, Over-Expression

This is an AAV expressing Human DBT.

AAV-206629
AAV-h-DBT

Ready-to-use AAV expressing Human DBT (dihydrolipoamide branched chain transacylase E2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CMV (default), or choose from 30 different ubiquitous or cell specific promoter
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
BCATE2; BCKAD-E2; BCKADE2; BCOADC-E2; E2; E2B
Description (Vector)
The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.
Gene ID
1629
Gene Name (Vector)
dihydrolipoamide branched chain transacylase E2
Gene Symbol
DBT
HGNC ID
HGNC:2698
ORF Size (aa)
1449
ORF Size (bp)
1449 bp
RefSeq ID
NM_001918
RefSeq Synonyms
NM_001918, BC016675,
Species
human
UniGene ID
Hs.709187

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.