human BTRC shRNA silencing AAV

Name
AAV-h-BTRC-shRNA

Cat No
shAAV-202354

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human BTRC.

shAAV-202354

AAV-h-BTRC-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human BTRC (beta-transducin repeat containing E3 ubiquitin protein ligase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

BETA-TRCP; betaTrCP; bTrCP; bTrCP1; FBW1A; FBXW1; FBXW1A; FWD1

Description (Vector)

BTRC encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. The encoded protein mediates degradation of CD4 via its interaction with HIV-1 Vpu. It has also been shown to ubiquitinate phosphorylated NFKBIA (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha), targeting it for degradation and thus activating nuclear factor kappa-B. Alternatively spliced transcript variants have been described. A related pseudogene exists in chromosome 6.

Gene ID

8945

Gene Name (Vector)

beta-transducin repeat containing E3 ubiquitin protein ligase

Gene Symbol

BTRC

HGNC ID

HGNC:1144

ORF Size (aa)

1818

ORF Size (bp)

1818 bp

RefSeq ID

NM_003939

RefSeq Synonyms

NM_033637, NM_003939, NM_001256856, BC027994,

Species

human

UniGene ID

Hs.643802

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.