human BTD shRNA silencing AAV

Name 
AAV-h-BTD-shRNA
Cat No 
shAAV-202323
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human BTD.

shAAV-202323
AAV-h-BTD-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human BTD (biotinidase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Description (Vector)
Biotinidase functions to recycle biotin in the body by cleaving biocytin (biotin-epsilon-lysine), a normal product of carboxylase degradation, resulting in regeneration of free biotin. Biotinidase has also been shown to have biotinyl-transferase activity. Defects in the biotinidase gene cause multiple carboxylase deficiency.
Gene ID
686
Gene Name (Vector)
biotinidase
Gene Symbol
BTD
HGNC ID
HGNC:1122
ORF Size (aa)
1632
ORF Size (bp)
1632 bp
RefSeq ID
NM_000060
RefSeq Synonyms
NM_001323582, NM_001281726, NM_001281725, NM_001281724, NM_001281723, NM_000060, BC012099,
Species
human
UniGene ID
Hs.517830

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About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.