human BRD4 shRNA silencing AAV

Name 
AAV-h-BRD4-shRNA
Cat No 
shAAV-202257
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human BRD4.

shAAV-202257
AAV-h-BRD4-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human BRD4 (bromodomain containing 4). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
bromodomain-containing protein 4; CAP; chromosome-associated protein; HUNK1; HUNKI; MCAP; Protein HUNK1
Description (eCommerce)
Bromodomain-containing protein 4 (BRD4) contains 2 bromo-domains. Bromo-domains are found in chromatin-associating proteins and can interact specifically with acetylated lysine. BRD4 is described as a chromatin adaptor and has been shown to bind acetylated chromatin. It was originally identified as MCAP (mitotic chromosome associated protein) due to its association with mitotic chromosomes. BRD4 also associates with several transcription complexes such as the Mediator complexes and P-TEFb complexes and is considered to function as a transcriptional co-factor.
Description (Vector)
BRD4 is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.
Gene ID
23476
Gene Name (eCommerce)
bromodomain containing 4
Gene Name (Vector)
bromodomain containing 4
Gene Symbol
BRD4
HGNC ID
HGNC:13575
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2385
ORF Size (bp)
2385 bp
Protein Name (eCommerce)
Bromodomain-containing protein 4
RefSeq ID
NM_058243
RefSeq Synonyms
NM_058243, NM_014299, NM_001330384, BC035266,
Research Areas
Cell Cycle,DNA Damage/Repair,Histone Modification,Host-Virus Interactions,Transcription Factor/Regulator
Research Areas (Faceted)
cell_biology,cell_cycle,genetics,transcription_translation,infectious_disease
Species
human
Target Sentence
Bromodomain-containing protein 4 (BRD4) contains 2 bromo-domains. Bromo-domains are found in chromatin-associating proteins and can interact specifically with acetylated lysine. BRD4 is described as a chromatin adaptor and has been shown to bind acetylated chromatin. It was originally identified as MCAP (mitotic chromosome associated protein) due to its association with mitotic chromosomes. BRD4 also associates with several transcription complexes such as the Mediator complexes and P-TEFb complexes and is considered to function as a transcriptional co-factor.
UniGene ID
Hs.187763
UniProt ID (eCommerce)
O60885

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.