human BLM shRNA silencing AAV

Name 
AAV-h-BLM-shRNA
Cat No 
shAAV-202164
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human BLM.

shAAV-202164
AAV-h-BLM-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human BLM (Bloom syndrome RecQ like helicase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
Bloom syndrome protein; Bloom syndrome RecQ like helicase; Bloom syndrome, RecQ helicase-like; BS; DNA helicase, RecQ-like type 2; MGRISCE1; recQ protein-like 3; RECQ2; RECQL2; RECQL3
Description (eCommerce)
Bloom syndrome protein (BLM) is a RecQ DNA-helicase that plays a role in DNA replication and repair. Mutations and inactivation of BLM result in human syndromes characterized by dwarfism, infertility, erythema, learning disabilities, immune deficiency and predisposition to cancer. BLM has been shown to associate with the BRCA1-associated genome surveillance complex (BASC) which contains BRCA1, MSH2, MSH6, MLH1, ATM, PMS2 and the RAD50-MRE11-NBS1 protein complex.
Description (Vector)
The Bloom syndrome gene product is related to the RecQ subset of DExH box-containing DNA helicases and has both DNA-stimulated ATPase and ATP-dependent DNA helicase activities. Mutations causing Bloom syndrome delete or alter helicase motifs and may disable the 3'-5' helicase activity. The normal protein may act to suppress inappropriate recombination.
Gene ID
641
Gene Name (eCommerce)
BLM RecQ like helicase
Gene Name (Vector)
Bloom syndrome RecQ like helicase
Gene Symbol
BLM
HGNC ID
HGNC:1058
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
3129
ORF Size (bp)
3129 bp
Protein Name (eCommerce)
Bloom syndrome protein
RefSeq ID
NM_000057
RefSeq Synonyms
NM_001287248, NM_001287247, NM_001287246, NM_000057, BC143280, BC115032, BC115030, BC101567, BC093622,
Research Areas
Breast Cancer,Cancer,Cell Cycle,DNA Damage/Repair,DNA Replication,Fanconi anemia,Helicase Activity,Homologous Recombination,Immunology,Prostate Cancer,Replication,Telomeric Maintenance,Tumor Suppressors/Oncoproteins
Research Areas (Faceted)
cancer,cell_biology,cell_cycle,genetics,transcription_translation,cell_biology,cell_cycle,immunology,cell_biology,cell_cycle,genetics,cancer,transcription_translation
Species
human
Target Sentence
Bloom syndrome protein (BLM) is a RecQ DNA-helicase that plays a role in DNA replication and repair. Mutations and inactivation of BLM result in human syndromes characterized by dwarfism, infertility, erythema, learning disabilities, immune deficiency and predisposition to cancer. BLM has been shown to associate with the BRCA1-associated genome surveillance complex (BASC) which contains BRCA1, MSH2, MSH6, MLH1, ATM, PMS2 and the RAD50-MRE11-NBS1 protein complex.
UniGene ID
Hs.716515
UniProt ID (eCommerce)
P54132

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.