human ATP6V1E1 shRNA silencing AAV

Name
AAV-h-ATP6V1E1-shRNA

Cat No
shAAV-201765

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human ATP6V1E1.

shAAV-201765

AAV-h-ATP6V1E1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human ATP6V1E1 (ATPase H+ transporting V1 subunit E1). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

ATP6E; ATP6E2; ATP6V1E; P31; Vma4

Description (Vector)

ATP6V1E1 encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. ATP6V1E1 encodes alternate transcriptional splice variants, encoding different V1 domain E subunit isoforms. Pseudogenes for this gene have been found in the genome.

Gene ID

529

Gene Name (Vector)

ATPase H+ transporting V1 subunit E1

Gene Symbol

ATP6V1E1

HGNC ID

HGNC:857

ORF Size (aa)

681

ORF Size (bp)

681 bp

RefSeq ID

NM_001696

RefSeq Synonyms

NM_001696, NM_001039367, NM_001039366, BC004443,

Species

human

UniGene ID

Hs.517338

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.