human ARNT shRNA silencing AAV

Name 
AAV-h-ARNT-shRNA
Cat No 
shAAV-201450
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human ARNT.

shAAV-201450
AAV-h-ARNT-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human ARNT (aryl hydrocarbon receptor nuclear translocator). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
ARNT protein; aryl hydrocarbon receptor nuclear translocator; bHLHe2; class E basic helix-loop-helix protein 2; dioxin receptor, nuclear translocator; HIF1B; HIF1BETA; HIF-1beta; HIF-1-beta; HIF1-beta; hypoxia-inducible factor 1, beta subunit; Hypoxia-inducible factor 1-beta; TANGO
Description (eCommerce)
Aryl hydrocarbon receptor nuclear translocator (ARNT) is involved in the induction of several enzymes that participate in xenobiotic metabolism. The ligand-free, cytosolic form of the Ah receptor is complexed to heat shock protein 90. Binding of ligand, which includes dioxin and polycyclic aromatic hydrocarbons, results in translocation of the ligand-binding subunit only to the nucleus. Induction of enzymes involved in xenobiotic metabolism occurs through binding of the ligand-bound Ah receptor to xenobiotic responsive elements in the promoters of genes for these enzymes. ARNT forms a complex with the ligand-bound Ah receptor, and is required for receptor function. ARNT has also been identified as the beta subunit of a heterodimeric transcription factor, hypoxia-inducible factor 1 (HIF1). A t(1;12)(q21;p13) translocation, which results in a TEL-ARNT fusion protein, is associated with acute myeloblastic leukemia [taken from NCBI Entrez Gene (GeneID: 405)].
Description (Vector)
The aryl hydrocarbon (Ah) receptor is involved in the induction of several enzymes that participate in xenobiotic metabolism. The ligand-free, cytosolic form of the Ah receptor is complexed to heat shock protein 90. Binding of ligand, which includes dioxin and polycyclic aromatic hydrocarbons, results in translocation of the ligand-binding subunit only to the nucleus. Induction of enzymes involved in xenobiotic metabolism occurs through binding of the ligand-bound Ah receptor to xenobiotic responsive elements in the promoters of genes for these enzymes. This gene encodes a protein that forms a complex with the ligand-bound Ah receptor, and is required for receptor function. The encoded protein has also been identified as the beta subunit of a heterodimeric transcription factor, hypoxia-inducible factor 1. A t(1;12)(q21;p13) translocation, which results in a TEL-ARNT fusion protein, is associated with acute myeloblastic leukemia. Alternative splicing results in multiple transcript variants.
Gene ID
405
Gene Name (eCommerce)
aryl hydrocarbon receptor nuclear translocator
Gene Name (Vector)
aryl hydrocarbon receptor nuclear translocator
Gene Symbol
ARNT
HGNC ID
HGNC:700
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1251
ORF Size (bp)
1251 bp
Protein Name (eCommerce)
Aryl hydrocarbon receptor nuclear translocator
RefSeq ID
NM_001668
RefSeq Synonyms
NM_178427, NM_001668, NM_001286036, NM_001286035, NM_001197325, BC060838,
Research Areas
Angiogenesis,Cancer,Cardiovascular,Gene Expression,HIF-1 Signaling,Hypoxia,Kidney Cancer,Metabolism/Metabolic Process,Neurobiology,Signal Transduction,Transcription Factor/Regulator
Research Areas (Faceted)
cancer,cardiovascular,cell_biology,developmental_biology,metabolism,neurobiology,signal_transduction,transcription_translation
Species
human
Target Sentence
Aryl hydrocarbon receptor nuclear translocator (ARNT) is involved in the induction of several enzymes that participate in xenobiotic metabolism. The ligand-free, cytosolic form of the Ah receptor is complexed to heat shock protein 90. Binding of ligand, which includes dioxin and polycyclic aromatic hydrocarbons, results in translocation of the ligand-binding subunit only to the nucleus. Induction of enzymes involved in xenobiotic metabolism occurs through binding of the ligand-bound Ah receptor to xenobiotic responsive elements in the promoters of genes for these enzymes. ARNT forms a complex with the ligand-bound Ah receptor, and is required for receptor function. ARNT has also been identified as the beta subunit of a heterodimeric transcription factor, hypoxia-inducible factor 1 (HIF1). A t(1;12)(q21;p13) translocation, which results in a TEL-ARNT fusion protein, is associated with acute myeloblastic leukemia [taken from NCBI Entrez Gene (GeneID: 405)].
UniGene ID
Hs.632446
UniProt ID (eCommerce)
P27540

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.