human ALDOA shRNA silencing AAV

Name
AAV-h-ALDOA-shRNA

Cat No
shAAV-200753

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human ALDOA.

shAAV-200753

AAV-h-ALDOA-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human ALDOA (aldolase, fructose-bisphosphate A). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

ALDA; aldolase A, fructose-bisphosphate; epididymis secretory sperm binding protein Li 87p; fructose-1,6-bisphosphate triosephosphate-lyase; fructose-bisphosphate aldolase A; GSD12; HEL-S-87p; lung cancer antigen NY-LU-1; muscle-type aldolase

Description (eCommerce)

Aldolase A (fructose-bisphosphate aldolase) (ALDOA) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia [taken from NCBI Entrez Gene (Gene ID: 226)].

Description (Vector)

ALDOA, Aldolase A (fructose-bisphosphate aldolase), is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing and alternative promoter usage results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 3 and 10.

Gene ID

226

Gene Name (eCommerce)

aldolase, fructose-bisphosphate A

Gene Name (Vector)

aldolase, fructose-bisphosphate A

Gene Symbol

ALDOA

HGNC ID

HGNC:414

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

1095

ORF Size (bp)

1095 bp

Protein Name (eCommerce)

Fructose-bisphosphate aldolase A

RefSeq ID

NM_000034

RefSeq Synonyms

NM_184043, NM_184041, NM_001243177, NM_001127617, NM_000034, BC016800, BC015888, BC013614, BC012880, BC010660, BC004333,

Research Areas

Breast Cancer,Cancer,Cytoskeleton,EGFR Signaling,HIF-1 Signaling,Metabolism/Metabolic Process,Signal Transduction

Research Areas (Faceted)

cancer,cell_biology,metabolism,signal_transduction

Species

human

Target Sentence

UniGene ID

Hs.513490

UniProt ID (eCommerce)

P04075

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.