human ACHE shRNA silencing AAV

Name
AAV-h-ACHE-shRNA

Cat No
shAAV-200189

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human ACHE.

shAAV-200189

AAV-h-ACHE-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human ACHE (acetylcholinesterase (Cartwright blood group)). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

ACEE; ARACHE; N-ACHE; YT

Description (Vector)

Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally.

Gene ID

Gene Name (Vector)

acetylcholinesterase (Cartwright blood group)

Gene Symbol

ACHE

HGNC ID

HGNC:108

ORF Size (aa)

1854

ORF Size (bp)

1854 bp

RefSeq ID

NM_000665

RefSeq Synonyms

NM_015831, NM_001302622, NM_001302621, NM_001282449, NM_000665, BC143469, BC105062, BC105060,

Species

human

UniGene ID

Hs.154495

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.