Fast Picks, Bench-Verified Wins, and Ready-Made shRNA
R&D teams routinely lose weeks to troubleshooting gene knockdown—juggling inconsistent clones, off-target effects, and ambiguous assay readouts. What you really need is decision-quality data, fast, without building an entire RNAi validation workflow in-house. VBL has multiple solutions rooted in science, speed, and quality. What is shRNA? “Short Hairpin RNA” is a small, specially designed piece of RNA that folds into a hairpin (loop) and is used to turn off or “silence” specific genes inside cells—think of it as a targeted mute button for genes. It’s widely used to study what happens when a specific gene is turned off.
If your goal is reliable gene silencing, you’ve got three efficient routes—each tuned to where your project is today.
1) Computationally Designed shRNA — Speed & early triage
Use this when you need rapid, low-cost candidate sequences to answer “Is this target likely silenceable?” We algorithmically design and rank shRNA against your transcripts (with isoform/UTR options and off-target minimization) so you can triage multiple targets, seed pilots, and de-risk before wet-lab work or viral packaging (AAV/Adenovirus).
Best for: Early discovery, budget-sensitive programs, or teams planning in-house testing.
Outcome: Fast answers if a gene can be silenced.
Upgrade path: Promote into our full validation services for in depth results.
2) Design, Screening & Validation — End to end data
Choose this when you need evidence, not predictions. We synthesize and test five candidates per target in cultured cells using a dual-luciferase assay (gLuc normalization + cLuc functional readout), then deliver the best performer(s) with clear results and guidance for qPCR/protein confirmation—ready to package into AAV or Adenovirus.
Best for: Program gates, partner reviews, hard models, or when prior RNAi attempts were inconsistent.
Outcome: A validated shRNA you can move forward with confidence.
3) Where Pre-validated shRNA — Trusted authority
Pre-validated shRNA (often TRC clones under a U6 promoter) are ready-made hairpins already shown to knock down specific genes in standard cultured conditions. They’re ideal when your cell system resembles common validation settings, the transcript is straightforward (limited isoforms), or you need a trusted benchmark right away. Use them to jump-start experiments, establish a positive control, or baseline performance before custom work. If results shift in your model, escalate to Design, Screening & Validation to optimize in context.
Best for: Benchmarking, rapid program start.
Outcome: Cost-effective gene silencing.
Quick Guide
- Need fast candidates? Start with Computational Design.
- Need defendable, packaging-ready results? Choose Design, Screening & Validation.
- Want a proven benchmark today? Use Pre-Validated shRNA and confirm in your system.
We are ready to help with your gene silencing project!
Current Offer
- 20% OFF Computationally Designed shRNA (in-silico only).
- 10% OFF Full Design, Screening, and Validation Services and Pre-Validated shRNA.
Ask us about combining tiers to go from fast picks to bench-verified knockdown with AAV/Adenovirus packaging.
About Vector Biolabs
Vector Biolabs’ cutting-edge viral vector technologies support a wide range of critical applications in biomedical research and therapeutic development. From advancing gene therapy treatments for genetic disorders to validating drug targets with precision, our vectors empower researchers to achieve reliable and impactful results.
If you want to know more about pre-validated shRNA contact us.