AAV with ELA1 promoter driven ECFP

Name
AAV-ELA1-ECFP-WPRE

Cat No
VB5322

Availability
3 weeks

Categories
AAV, Control/Reporter, Over-Expression

This AAV expresses ECFP driven by a pancreatic acinar ELA1 promoter.

Rat elastase 1 (Ela1; ELAI1; RATELAI1), also called chymotrypsin-like elastase family, member 1, is the major protease with elastin-degrading activity in the mammalian exocrine pancreas. The ELA1 promoter (0.3Kb) is comprised of the core promoter and enhancer, and directs transgene expression in pancreatic acinar cells, stomach, duodenum, and colon.

Enhanced Cyan fluorescence protein(ECFP) is a cyan variant from the Aequorea GFP, with a Excitation/Emission spectrum around 434nm/477nm. Other versions of fluorescent proteins with similar excitation and emission spectrums include mcerulean3, mTurquoise, and SCFP.

VB5322

AAV-ELA1-ECFP-WPRE

Ready-to-use AAV expressing ECFP driven by a pancreatic acinar ELA1 promoter. Available in AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ and other serotypes.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

ELA1 (pancreatic acinar)

Storage Buffer

PBS/5% Glycerol

Titer

1x10^13 GC/ml

Viral Backbone

Recombinant AAV

Volume (µL)

200

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.