AAV with MBP promoter driven eArch3.0-Venus

This AAV expresses eArch3.0-Venus driven by an olgiodendricyte MBP promoter.

The myelin basic proteins (MBPs) are a family of polypeptides that are predominantly expressed in the nervous system, where they play a major role in myelination. This 1.3 Kb mouse MBP promoter drives reporter gene expression mainly in oligodendrocytes.

Arch and ArchT are light-driven proton pumps found in archaebacteria. They provide silencing via hyperpolarization. Only slightly slower than NpHR2, their peak activation falls at 566 nm. ArchT has about a three-fold higher light sensitivity compared to Arch leading to a similar level of inhibition but increased volume of tissue that will be inhibited.

The trafficking signal (KSRITSEGEYIPLDQIDINV) is also from the inward rectifier potassium channel Kir2.1; it serves to dramatically reduce intracellular accumulation and improve membrane targeting, leading to a profound increase of photocurrents. This is the basis for many third-generation optogenetics tools.

This ER export motif (FCYENEV) is from the vertebrate inward rectifier potassium channel Kir2.1. The motif prevents aggregate formation, decreases intracellular accumulations, and enhances tolerability at high expression levels. This is the strategy for developing many second-generation optogenetics tools.