AAV with CMV7 promoter driven VChR1-ECFP

Name
AAV-CMV7-VChR1-ECFP

Cat No
VB3915

Availability
3 weeks

Categories
Optogenetics, AAV, Over-Expression

This AAV expresses VChR1-ECFP driven by an ubiquitous CMV7 promoter.

The CMV7 promoter is about 0.35 kb in length and is a shortened version of the commonly used CMV promoter. CMV7 has a truncated version of the CMV enhancer. When tested in several cell lines, this promoter provides an expression level that is about 3-5 fold lower than that of the original 0.6Kb CMV promoter.

Volvox channelrhodopsin-1 (VChR1) is from the multicellular green alga Volvox carteri. VChR1 has a similar functionality as hChR2 but with a different excitation spectrum towards green light, and shows action potential firing with 589 nm light.

VB3915

AAV-CMV7-VChR1-ECFP

Ready-to-use AAV expressing VChR1-ECFP driven by an ubiquitous CMV7 promoter. Available in AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ and other serotypes.

Request a Quote

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

CMV7 (ubiquitous)

Storage Buffer

PBS/5% Glycerol

Titer

1x10^13 GC/ml

Viral Backbone

Recombinant AAV

Volume (µL)

200

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.