AAV with CMV-IVS promoter driven VChR1-ECFP

Name 
AAV-CMVIVS-VChR1-ECFP
Cat No 
VB3657
Availability
3 weeks
Categories
Optogenetics, AAV, Over-Expression

This AAV expresses VChR1-ECFP driven by an ubiquitous CMV-IVS promoter.

The CMV-IVS promoter is about 1.1 kb in length and consists of the commonly used CMV promoter/enhancer and the intron (IVS) from beta-globin . The presence of intron elements was shown to give a higher level of transgene expression than that of the original CMV promoter/enhancer.

Volvox channelrhodopsin-1 (VChR1) is from the multicellular green alga Volvox carteri. VChR1 has a similar functionality as hChR2 but with a different excitation spectrum towards green light, and shows action potential firing with 589 nm light.

VB3657
AAV-CMVIVS-VChR1-ECFP

Ready-to-use AAV expressing VChR1-ECFP driven by an ubiquitous CMV-IVS promoter. Available in AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ and other serotypes.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CMV-IVS (ubiquitous)
Storage Buffer
PBS/5% Glycerol
Titer
1x10^13 GC/ml
Viral Backbone
Recombinant AAV
Volume (µL)
200

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.