AAV with CMV promoter driven eArchT3.0-ECFP

This AAV expresses eArchT3.0-ECFP driven by an ubiquitous CMV promoter.

This roughly 0.6 Kb promoter is the commonly used human cytomegalovirus (CMV) immediate-early promoter/enhancer (CMV/IE).

Arch and ArchT are light-driven proton pumps found in archaebacteria. They provide silencing via hyperpolarization. Only slightly slower than NpHR2, their peak activation falls at 566 nm. ArchT has about a three-fold higher light sensitivity compared to Arch leading to a similar level of inhibition but increased volume of tissue that will be inhibited.

The trafficking signal (KSRITSEGEYIPLDQIDINV) is also from the inward rectifier potassium channel Kir2.1; it serves to dramatically reduce intracellular accumulation and improve membrane targeting, leading to a profound increase of photocurrents. This is the basis for many third-generation optogenetics tools.

This ER export motif (FCYENEV) is from the vertebrate inward rectifier potassium channel Kir2.1. The motif prevents aggregate formation, decreases intracellular accumulations, and enhances tolerability at high expression levels. This is the strategy for developing many second-generation optogenetics tools.