AAV with CAG2 promoter driven hChR2(C128S/D156A)-TdTomato

Name 
AAV-CAG2-hChR2(C128S/D156A)-TdTomato
Cat No 
VB2753
Availability
3 weeks
Categories
Optogenetics, AAV, Over-Expression

This AAV expresses hChR2(C128S/D156A)-TdTomato driven by an ubiquitous CAG2 promoter.

The CAG2 promoter is derived from the commonly used 1.8kb CAG/CBA (also called CAGGS) promoter. To increase the cloning capacity of size-limiting vectors using the CAG promoter, such as AAV or lentiviral vectors, CAG2 was developed by deleting a portion of about 0.6Kb of the chicken beta-actin intron from the original CAG promoter.

hChR2 is a humanized version of ChR2 for mammalian expression. Wild-type ChR2, as well as a few of its mutations, provides the fastest excitation of the channelrhodopsins offered, and is widely used in in optogenetics techniques in neuroscience. hChR2(D156) mutant is another SFO mutation with a 6.9-minute open-state lifetime, and hChR2(C128S/D156A) double mutation creates a Stabilized Step Function Opsin (SSFO) with a stability of the active state up to 30 minutes.

VB2753
AAV-CAG2-hChR2(C128S/D156A)-TdTomato

Ready-to-use AAV expressing hChR2(C128S/D156A)-TdTomato driven by an ubiquitous CAG2 promoter. Available in AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ and other serotypes.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CAG2 (ubiquitous)
Storage Buffer
PBS/5% Glycerol
Titer
1x10^13 GC/ml
Viral Backbone
Recombinant AAV
Volume (µL)
200

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.