AAV with ApoE/hAAT1 promoter driven TdTomato

Name
AAV-ApoE/hAAT1-TdTomato

Cat No
VB1965

Availability
2-3 weeks

Categories
AAV, Control/Reporter, Over-Expression

This AAV expresses TdTomato driven by a liver ApoE/hAAT1 promoter.

The ApoE/AAT1 chimeric promoter consists of the core promoter from human alpha-1-antitrypsin gene (also known as hAAT1, SERPINA1, A1A, A1AT, AAT, or alpha1AT) and the enhancer/hepatic locus control region (HCR) from the ApoE gene. The ApoE-hAAT1 chimeric promoter is about 0.7Kb and is used for driving gene expression in hepatocytes

dTomato belongs to a family of fluorescent proteins called mFruit. Two dTomato units were joined head-to-tail through a linker, yielding a tandem dimer fluorescent protein, tdTomato. The tdTomato derivative is the brightest fluorescent protein yet reported, featuring an orange-red emission maximum at 581nm and is one of the most photo-stable fluorescent proteins. Other fluorescent proteins with similar emission spectra include DsRed, DsRed2, TurboRFP, TagRFP, and TagRFP-T.

VB1965

AAV-ApoE/hAAT1-TdTomato

Ready-to-use AAV expressing TdTomato driven by a liver ApoE/hAAT1 promoter. Available in AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ and other serotypes.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

ApoE/hAAT1 (liver)

Storage Buffer

PBS/5% Glycerol

Titer

1x10^13 GC/ml

Viral Backbone

Recombinant AAV

Volume

100 µl, 250 µl, 500 µl, and 1ml

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.