rat NGLY1 shRNA silencing Adenovirus

Name
Ad-r-NGLY1-shRNA

Cat No
shADV-293592

Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Rat Ngly1.

shADV-293592

Ad-r-NGLY1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Rat Ngly1 (N-glycanase 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

shRNA Validated

Yes

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Description (Vector)

Specifically deglycosylates the denatured form of N-linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl-glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high-mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulun that are exported in the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins.

Gene ID

361014

Gene Name (Vector)

N-glycanase 1

Gene Symbol

Ngly1

ORF Size (aa)

1812

ORF Size (bp)

1812 bp

RefSeq ID

NM_001014136

RefSeq Synonyms

NM_001014136, BC083837,

Species

rat

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.