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rat SF3B1 shRNA silencing Adenovirus

Name 
Ad-r-SF3B1-shRNA
Cat No 
shADV-292921
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Rat Sf3b1.

shADV-292921
Ad-r-SF3B1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Rat Sf3b1 (splicing factor 3b, subunit 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
50
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
Sap155
Description (Vector)
Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.
Gene ID
84486
Gene Name (Vector)
splicing factor 3b, subunit 1
Gene Symbol
Sf3b1
ORF Size (aa)
225
RefSeq ID
NM_053426
RefSeq Synonyms
NM_053426, BC089925,
Species
rat

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.