rat UIMC1 shRNA silencing Adenovirus

Name
Ad-r-UIMC1-shRNA

Cat No
shADV-292529

Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Rat Uimc1.

shADV-292529

Ad-r-UIMC1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Rat Uimc1 (ubiquitin interaction motif containing 1). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

shRNA Validated

Yes

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

RGD1307009

Description (Vector)

Ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly Act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1.

Gene ID

290997

Gene Name (Vector)

ubiquitin interaction motif containing 1

Gene Symbol

Uimc1

ORF Size (aa)

2181

ORF Size (bp)

2181 bp

RefSeq ID

NM_001013884

RefSeq Synonyms

NM_001013884, BC087149,

Species

rat

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.