rat NCL shRNA silencing Adenovirus

Name 
Ad-r-NCL-shRNA
Cat No 
shADV-292150
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Rat Ncl.

shADV-292150
Ad-r-NCL-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Rat Ncl (nucleolin). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
98
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Description (Vector)
Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
Gene ID
25135
Gene Name (Vector)
nucleolin
Gene Symbol
Ncl
ORF Size (aa)
2145
ORF Size (bp)
2145 bp
RefSeq ID
NM_012749
RefSeq Synonyms
NM_012749, BC085751,
Species
rat

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.