rat TMEM66 shRNA silencing Adenovirus

Name
Ad-r-TMEM66-shRNA

Cat No
shADV-291051

Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Rat Saraf.

shADV-291051

Ad-r-TMEM66-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Rat Saraf (store-operated calcium entry-associated regulatory factor). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

shRNA Validated

Yes

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

Tmem66

Description (Vector)

Negative regulator of store-operated Ca(2+) entry (SOCE) involved in protecting cells from Ca(2+) overfilling. In response to cytosolic Ca(2+) elevation after endoplasmic reticulum Ca(2+) refilling, promotes a slow inactivation of STIM (STIM1 or STIM2)-dependent SOCE activity: possibly act by facilitating the deoligomerization of STIM to efficiently turn off ORAI when the endoplasmic reticulum lumen is filled with the appropriate Ca(2+) levels, and thus preventing the overload of the cell with excessive Ca(2+) ions.

Gene ID

290796

Gene Name (Vector)

store-operated calcium entry-associated regulatory factor

Gene Symbol

Saraf

ORF Size (aa)

1005

ORF Size (bp)

1005 bp

RefSeq ID

NM_001004213

RefSeq Synonyms

NM_001004213, BC078979,

Species

rat

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.