rat TRIM25 shRNA silencing Adenovirus

Name
Ad-r-TRIM25-shRNA

Cat No
shADV-290363

Availability
7-8 weeks

This is an Adenovirus expressing shRNA for silencing of Rat Trim25.

shADV-290363

Ad-r-TRIM25-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Rat Trim25 (tripartite motif-containing 25). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Validated

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

Efp; Znf147

Description (Vector)

Functions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs.

Gene ID

494338

Gene Name (Vector)

tripartite motif-containing 25

Gene Symbol

Trim25

ORF Size (aa)

1932

ORF Size (bp)

1932 bp

RefSeq ID

NM_001009536

RefSeq Synonyms

NM_001009536, BC061749,

Species

rat

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.