mouse USP28 shRNA silencing Adenovirus
This is an Adenovirus expressing shRNA for silencing of Mouse Usp28.
shADV-281197
Ad-m-USP28-shRNA
Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Usp28 (ubiquitin specific peptidase 28). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
9830148O20Rik; AU022237; mKIAA1515
Description (Vector)
Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus.
Gene ID
235323
Gene Name (Vector)
ubiquitin specific peptidase 28
Gene Symbol
Usp28
ORF Size (aa)
3156
ORF Size (bp)
3156 bp
RefSeq ID
NM_175482
RefSeq Synonyms
NM_175482, BC088733, BC066033,
Species
mouse
UniGene ID
Mm.21630
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.