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mouse UBE2F shRNA silencing Adenovirus

Name 
Ad-m-UBE2F-shRNA
Cat No 
shADV-275474
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Ube2f.

shADV-275474
Ad-m-UBE2F-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Ube2f (ubiquitin-conjugating enzyme E2F (putative)). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
2510010F15Rik; AI851109
Description (Vector)
Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.
Gene ID
67921
Gene Name (Vector)
ubiquitin-conjugating enzyme E2F (putative)
Gene Symbol
Ube2f
ORF Size (aa)
558
ORF Size (bp)
558 bp
RefSeq ID
NM_026454
RefSeq Synonyms
NM_026454, BC016117,
Species
mouse
UniGene ID
Mm.337238

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.