mouse TCFE2A shRNA silencing Adenovirus

Name 
Ad-m-TCFE2A-shRNA
Cat No 
shADV-273939
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Tcf3.

shADV-273939
Ad-m-TCFE2A-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Tcf3 (transcription factor 3). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
A1; AA408400; ALF2; AW209082; bHLHb21; E12; E12/E47; E2A; E47; KA1; ME2; Pan1; Pan2; TCF-3; Tcfe2a; VDIR
Description (Vector)
Transcriptional regulator. Involved in the initiation of neuronal differentiation. Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Binds to IEB1 and IEB2, which are short DNA sequences in the insulin gene transcription control region.
Gene ID
21423
Gene Name (Vector)
transcription factor 3
Gene Symbol
Tcf3
ORF Size (aa)
1947
ORF Size (bp)
1947 bp
RefSeq ID
NM_011548
RefSeq Synonyms
NM_011548, NM_001164153, NM_001164152, NM_001164151, NM_001164150, NM_001164149, NM_001164148, NM_001164147, BC018260, BC006860,
Species
mouse
UniGene ID
Mm.3406

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.